This technical brief explores strategies to enhance poultry biosecurity in Ashaiman Municipal District, Ghana. It highlights the significant growth of Ghana's poultry sector, driven by increased egg consumption and urbanization. The document highlights the importance of biosecurity in preventing disease outbreaks, such as avian influenza, and improving farm productivity and profitability. It also examines the challenges faced by poultry farmers and public veterinary services, offering recommendations for sustainable biosecurity practices through public–private partnerships. The brief aims to support the implementation of the FAO Progressive Management Pathway for Terrestrial Animal Biosecurity (FAO-PMP-TAB) in Ghana, fostering a resilient and thriving poultry sector.